Mouse anti-Human IgM Antibody

Catalog No. ABIN135610

Quick Overview for Mouse anti-Human IgM Antibody (ABIN135610)

Target: IgM

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Clone: SA-DA4

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Specificity: Reacts with the heavy chain of human IgM as demonstrated by ELISA.

Characteristics: Mouse Anti-Human IgM-UNLB

Purification: Purified

Immunogen: Human IgM myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Stim - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE, APC, and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of B cells

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: IgM

Target Type: Antibody